![]() The backcross-derived lines gave a higher yield under moisture-deficit stress conditions compared with the recipient parent. This study resulted in 23 improved lines carrying combinations of four drought tolerance QTLs with a range of 85.35%–95.79% background recovery. Marker-assisted selection coupled with stringent phenotypic screening was used to advance each generation. In the current investigation, we transferred four drought tolerance quantitative trait loci (QTLs) controlling traits, viz canopy temperature, normalized difference vegetative index, chlorophyll content, and grain yield from the drought-tolerant donor line, C306, into a popular high-yielding, drought-sensitive variety, HD2733. ![]() Marker-assisted backcross breeding enables selective insertion of targeted traits into the genome to improve yield, quality, and stress resistance in wheat. 6PPV & FR Authority NAAS complex, New Delhi, India.5ICAR-Indian wheat and Barley Research Institute, Karnal, India.4ICAR-Indian Grassland and Fodder Research Institute, Jhansi, India.3ICAR-Indian Institute of Oilseed Research, Hyderabad, India.2ICAR-Indian Institute Millets Research, Hyderabad, India.1ICAR-Indian Agricultural Research Institute, New Delhi, India.Singh 1, Gyanendra Pratap Singh 5 and Kumble Vinod Prabhu 6* Shashikumara 4, Nivedita Sinha 1, Neelu Jain 1, Pradeep K. Kumar 1, Amasiddha Bellundagi 2, Hari Krishna 1*, Mallana Gowdra Mallikarjuna 1, Ramya K.
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